ced Rat Model for Breast Cancer

nloaded purpose of this investigation was to identify pathways by which physical activity, implemented as g in an activity wheel, inhibits carcinogenesis. The focus of this analysis was on 20 plasma biomarr glucose homeostasis, inflammation, cytokine function, and endocrine activity, known to be afby a physically active lifestyle. Plasma for analysis was obtained from previously reported ogenesis experiments in which the effects on mammary carcinogenesis, induced by i.p. injection ethyl-1-nitrosurea, of running on a motorized activity wheel or a nonmotorized free wheel were ared with sedentary controls. Wheel running reduced cancer incidence (P = 0.0004) and the number cers per animal (P = 0.005). Principal components analysis was used to reduce the 20 plasma biomara concise index that was significantly different by treatment group assignment (P < 0.0001). Statistical es provided evidence that supported the hypothesis of amediational role of thesemolecules in accountr the protective effect of wheel running on the carcinogenic process. In addition, the plasma biomarker derived from principal components analysis was a good discriminator of treatment group assignment 4.5% of animals were misclassified). These findings suggest that the plasma biomarkers evaluated have in assessing the breast cancer response to a physical activity intervention. Identification of such bioutility markers is critical in clinical studies for which evaluating the effects of physical activity on cancer outcomes (diagnosis, recurrence, or mortality) is not possible. Cancer Prev Res; 3(11); 1484–92. ©2010 AACR.